Specializace

Industrial/Environmental Microbiology

Currently, we are working on millipede gut microbiome. Our interest in this area of research stems from the fact that millipedes play a major role in organic matter decomposition and nutrient recycling in terrestrial ecosystems. They are one of the most important detritivores consuming decaying wood, soil and leaf litter in various stages of decomposition. Like all other arthropods, millipedes host a diverse community of microbes in their guts, which is believed to aid in digestive processes and profoundly influence various aspects of their biology, yet the exact roles of this microbiome remain unclear. So, we using a combination of stable isotope probing (SIP), metagenomic, and metatranscriptomic techniques to understand the gut microbial community structure and functions.

Stručný životopis

Educational Background:

• PhD Student at the University of South Bohemia, Czech Republic (07/2019 - date)

• Exchange masters’ student at University of Tsukuba, Ibaraki, Japan (03/2016 - 02/2017).

• Master’s Degree (M. Sc.) in Industrial Microbiology (with CGPA of 4.29), University of Nigeria, Nsukka (2014 - 2016).

• National Youth Service Corps Certificate, Bayelsa State, Nigeria (2012 - 2013).

• Bachelor Degree (B. Sc.) (Second Class Upper Division with CGPA of 4.14) in Microbiology, University of Nigeria, Nsukka (2007 - 2011).

• Senior School Certificate Examination. Special Science School, Ukehe, Igbo Etiti L.G.A Enugu State (1998 - 2004).

• First School Leaving Certificate. Community Primary School, Olido, Enugu Ezike, Enugu State (1992 - 1998).

M.Sc. and B.Sc. Research Topics:

• Isolation and evaluation of thermotolerant-xylose fermenting yeasts for bioethanol production

• Alpha-amylase production from fungi isolated from rot cassava

Research Interests

• Microbial physiology, bioenergy/bioelectrochemistry, production of biochemicals, microbial interactions/community analysis, environmental microbiology, molecular biology and bioinformatics.

Published Research Papers

• Nweze Julius Eyiuche, Shiho Asakawa, Takahiro Yamashita, Atsuo Ikeguchi, Yutaka Kitamura and Hiroshi Yokoyama (2017). Community analysis of biofilms on flame-oxidized stainless steel anodes in microbial fuel cells fed with different substrates. BMC Microbiology, 17:145.

• Julius E. Nweze, Ifeanyi Ndubisi, Nnaemeka J. Onoyima et al. (2019). Isolation and evaluation of thermotolerant-xylose fermenting yeasts for bioethanol production. Biofuels (http://dx.doi.org/10.1080/17597269.2018.1564480)

• Charles O. Nwuche, Yoshinori Murata, Julius E. Nweze, Ifeanyi A. Ndubuisi, Hide Ohmae, Masayoshi Saito, James C. Ogbonna (2018). Bioethanol production under multiple stress condition by a new acid and temperature tolerant Saccharomyces cerevisiae strain LC 269108 isolated from rotten fruits. Process Biochemistry.

• Ifeanyi Ndubisi, Julius E. Nweze, Nnaemeka J. Onoyima et al. (2018). Ethanol production from cassava pulp by a newly isolated thermotolerant Pichia kudriavzevii LC375240. Energy and Power Engineering. 10(10); 457-474.

• Justus Amuche Nweze, Josephine I. Okafor, Emeka Innocent Nweze and Julius Eyiuche Nweze (2016). Comparison of antimicrobial potential of honey samples from Apis mellifera and two stingless bees from Nsukka, Nigeria. J Pharmacogn Nat Prod 2: 124.

• Justus Amuche Nweze, J. I. Okafor, Emeka I. Nweze and Julius Eyiuche Nweze (2017). Evaluation of physicochemical and antioxidant properties of two stingless bee honeys: a comparison with Apis mellifera honey from Nsukka, Nigeria. BMC Research Notes, 10:566.

International Conference/Symposium

• Poster presentation at Ag-ESD International Symposium held at Tsukuba University Japan (Nov. 2016)

Internship/Industrial Training

• Research assistant at National Agricultural and Food Research Organization (NARO), Tsukuba Ibaraki, Japan (01/08/2016-16/12/2016).

Awards and Scholarships

• Certificate of participation at Ag-ESD International Symposium held at Tsukuba University Japan (Nov. 2016).

• Outstanding poster award for a masters’ student for a poster presented at the Ag-ESD International Symposium held at Tsukuba University Japan (Nov. 2016).

• Japan Student Services Organization (JASSO) scholarship (03/2016-02/2017).

Další informace

Special Skills and Laboratory Techniques

Microbiology skills: Aseptic and sterile techniques, optical microscopy, bacterial staining, plating methods (streak, spread, pour, replica), enumeration and identification of bacteria, use of biological safety cabinets, media and buffer preparation, use of selective media, and antibiotic assay (MIC).

Molecular Biology skills: DNA extraction, plasmid DNA preparation, RNA isolation and purification, restriction enzyme digests, PCR, agarose and acrylamide gel electrophoresis, primer design, Southern blotting, Western blotting and Northern blotting.

Spectroscopy skills: UV and visible spectroscopy

Data analysis skills: Using packages: SPSS, R, QIIME, MEGA 7 and Molecular Biology software (NCBI and associated tools, and databases).

Computer Skills: Highly proficient in the use of Microsoft packages, Windows, Internet research, Blogging and mobile web designing.

Languages: English (fluent), Igbo (fluent), Japanese (elementary) and French (elementary)

Personal Skills: Hard working, disciplined, learning attitude, ability to work in teams as well as independently, ability to manage stress, sincere and self-confident, possess positive attitude, strong communication and interpersonal skills.

Activities and Hobbies: Football, blogging, mobile website designing, programming, reading and writing, travelling, teaching, listening to music, church work and exercise.