Outflows from two atmospherically acidified lakes in the Bohemian Forest were studied in 2005 and 2007. While Lake Certovo has been strongly acidified (similar to pH 4.6), Lake Laka was only slightly acidified in the past and is recovering now (similar to pH 5.2). The water chemistry and macrozoobenthos composition were analysed along longitudinal gradients of both lake outflows to determine the present status of their streams. A certain progression in stream chemistry to more neutral conditions was observed along the longitudinal gradients of both streams. However, a possible recovery of macrozoobenthos was evident only in the Lake Laka outflow, mainly via an increasing number of Ephemeroptera and Trichoptera taxa, and an increasing number of Gammarus fossarum, both along the longitudinal gradient and during the period of study. In contrast, no considerable changes were observed in the macrozoobenthos composition of the Lake Certovo outflow, presumably because its chemistry was harmful for acidosensitive taxa such as Ephemeroptera and Trichoptera. Plecoptera and Chironomidae were the most numerous groups in this stream. The biological recovery of both streams will depend on further chemical improvement in their catchments as well as on the dispersal ability of benthic organisms.