The chemoorganotrophic, aerobic, facultatively anaerobic, non-motile strain MWH-C5T isolated from the water column of oligo-mesotrophic Lake Mondsee (Austria) was phenotypically, phylogenetically, and chemotaxonomically characterized. The predominant fatty acids of the strain were C16:1{omega}7c/{omega}6c, C16:0, C12:1, and C8:0-3OH, the major quinone was ubiquinone Q-8, and the G+C content of the DNA of the strain was 55.5 mol%. The 16S rRNA gene similarity to the closest related type species were 96.6% (Curvibacter delicatus) and 95.7% (Rhodoferax fermentans). The phylogenetic analysis of the 16S rRNA gene sequences revealed the affiliation of the strain with the family Comamonadaceae (Betaproteobacteria), however, the revealed phylogenetic position of the strain did not indicate the affiliation to any previously described genus within this family. A family-wide comparison of traits revealed that the strain possesses a unique combination of G+C value, major fatty acids, and major 3-hydroxy fatty acid. Furthermore, the strain differs in several traits from the closest related genera. Based on the phylogeny of the strain and the differences to the closest related genera, we propose to establish the genus Limnohabitans gen. nov. to accommodate this strain, and to place the strain in the new species Limnohabitans curvus sp. nov., with the type strain MWH-C5T (DSM 21645T =CCUG 56720T). The type strain is closely related to a large number of uncultured bacteria detected by cultivation-independent methods in various freshwater systems.